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1Introduction

Many of the bacteria that inhabit chronic wounds set up complex polymicrobial biofilm communities

Many of the bacteria that inhabit chronic wounds set up complex polymicrobial biofilm communities that can only be detected by culture techniques when they happen to detach a sufficient bolus of planktonic cells that can be grown on conventional culture media.

A major problem in bony infections is the fact that biofilm bacteria cannot be recovered by standard culture techniques, which means that fracture repairs often yield negative culture results even when multiple clinical signs point to infection. New techniques such as sonication can increase the yield of the cultures. It is important to realize culture results are affected if the patient is being treated with antibiotics at the time of culture.